近年来革蓝氏阴性杆菌感染症逐年增多,氨基糖苷类抗生素的使用日益广泛和频繁。然而,这类抗生素对肾脏和第八脑神经有毒性,治疗量和中毒量较为接近。因此,很有必要根据测定的血浓度和药物动态理论设计投药方法。作者用HPLC、RIA、EIA-Ⅰ(EMIT)、EIA-Ⅱ(SLFIA)和生物检定法等5种方法测定0.45、1.05、2.00、3.98、7.94、13.16μg/ml等7个浓度的托普霉素血清溶液的浓度。此外,还测定由6名健康人和 In recent years, Gram-negative bacilli increased year by year, the use of aminoglycoside antibiotics is becoming more and more frequent. However, this kind of antibiotics is toxic to the kidneys and the eighth cranial nerve, and the amount of treatment and poisoning are relatively close. Therefore, it is necessary to design the method of administration based on the measured blood concentration and the pharmacokinetic theory. The authors used five methods including HPLC, RIA, EIA-I (EMIT), EIA-II (SLFIA) and bioassay methods to determine the concentrations of 0.80, 1.90, Serum concentration of serum solution. In addition, 6 healthy people were also tested