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研究载脂蛋白E基因敲除 (载脂蛋白E°)小鼠冠状动脉内粥样硬化病灶的分布、组成与动脉外膜炎症的关系。取载脂蛋白E°小鼠心脏作连续切片 ,Movat法染色 ,追踪冠状动脉主干及其心肌内的小分支 ;寻找病灶 ,观察病灶内组成 ,分析其分布规律。复制小鼠股动脉外膜无菌性炎症模型 ,用免疫组织化学方法检查内膜粘附分子的表达。结果发现 ,冠状动脉主干内有延伸病灶 ,在主干以下分支 (包括心肌内小分支 )内有在原位生成的病灶 ,在两类病灶相邻的外膜有炎性细胞浸润 ,外膜炎症面积大于动脉粥样硬化病灶累及的内膜面积 ,亦发现一些部位血管外有炎性细胞浸润 ,而尚无病灶形成。原位病灶均发生于心室壁 ,大的原位病灶多发生在左室壁心肌内、血管分支处和乳头肌附近的冠状动脉分支内。股动脉外膜炎症可诱发内膜表达细胞间粘附分子 1和血管细胞粘附分子 1,同时伴白细胞的附壁。以上提示 :血管外膜炎症是小鼠冠状动脉内病灶的一个始动环节。
To study the distribution and composition of atherosclerotic lesions in coronary arteries in the apolipoprotein E gene knockout (Apolipoprotein E) mice. The apolipoprotein E mouse hearts were sectioned and stained with Movat method to trace the small branches of the coronary artery and its myocardium. The lesion was observed and the distribution of lesions was observed. The model of aseptic inflammation of the femoral artery was replicated and the expression of adhesion molecules was detected by immunohistochemical method. The results showed that there are extended lesions within the trunk of the coronary artery in the trunk below the branches (including small branches within the heart) in situ generated lesions in two types of lesions adjacent to the outer membrane inflammatory cell infiltration, the outer membrane inflammation area Greater than atherosclerotic lesions involving the intima area, also found in some parts of extravascular inflammatory cell infiltration, but no lesions formed. In situ lesions occur in the ventricular wall, large in situ lesions occur in the left ventricular wall myocardium, vascular branches and near the branch of the coronary artery within the papillary muscle. Inflammatory femoral artery can induce intima expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1, accompanied by leukocyte adhesion. The above tips: Vascular adventitia is a coronary artery lesions in mice a starting point.