论文部分内容阅读
目的:通过机械和感染双重法构建一个犬的宫腔粘连动物模型,并检测血管内皮因子(vascular endothelial growth factor,VEGF)、转化生长因子(transforming growth factor-β1,TGF-β1)的表达,评估其与宫腔粘连形成的关系,为下一步研究提供实验平台。方法:选取6只性发育成熟的健康雌性中华田园犬,所有建模前的犬的一小段右侧宫角组织作为对照组,建模后该部位子宫角组织作为处理组。利用机械搔刮和感染双重法损伤右侧宫角内膜,于建模前后各收集一小段宫角标本,行HE、Masson染色及SP法免疫组化,整理并统计分析。结果:处理组的内膜厚度[(2.325±0.399)cm]薄于对照组[(3.025±0.466)cm],差异具有统计学意义(P=0.050);处理组的内膜腺体数目[(4.388±1.774)个]明显比对照组[(16.518±4.421)个]减少,差异具有统计学意义(P=0.001);处理组的子宫内膜纤维化面积比例(0.775±0.040)明显高于对照组(0.329±0.062),差异有统计学意义(P=0.000);处理组的子宫内膜中VEGF(5.967±0.390)、TGF-β1(6.200±0.306)的表达量高于对照组(1.700±0.823)、(1.100±0.361),差异均具有统计学意义(P=0.000,P=0.001)。结论:采用机械和感染双重法可以成功构建中华田园犬宫腔粘连的动物模型,且VEGF、TGF-β1的表达量升高可能与宫腔粘连的发生有关。
OBJECTIVE: To construct a canine intrauterine adhesions animal model by double-mechanics and infection methods and to detect the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) Its relationship with the formation of intrauterine adhesions for the next step to provide experimental platform. Methods: Six healthy female Chinese canines with sexual maturity were selected. A small section of right uterine tissue of all dogs before modeling was used as the control group. The uterine horn tissue of the part was treated as the treatment group. The right side of the endometrium was harmed by mechanical scraping and double infection. A small section of uterine horn specimens were collected before and after modeling. HE, Masson staining and SP immunohistochemistry were used for sorting and statistical analysis. Results: The intima thickness [(2.325 ± 0.399) cm] in the treated group was significantly lower than that in the control group [(3.025 ± 0.466) cm] (P = 0.050) 4.388 ± 1.774)] was significantly lower than that of the control group [(16.518 ± 4.421)], the difference was statistically significant (P = 0.001). The ratio of endometrial fibrosis area in the treatment group (0.775 ± 0.040) was significantly higher than that in the control group Group (0.329 ± 0.062), the difference was statistically significant (P = 0.000); The expression of VEGF (5.967 ± 0.390) and TGF-β1 (6.200 ± 0.306) in the endometrium of the treated group was higher than that of the control group (1.700 ± 0.823), (1.100 ± 0.361), the difference was statistically significant (P = 0.000, P = 0.001). Conclusion: The animal model of intrauterine adhesion of Chinese canine canine can be successfully established by the dual method of mechanical and infection. The increased expression of VEGF and TGF-β1 may be related to the intrauterine adhesions.