论文部分内容阅读
目的探讨葡萄内酯对快速老化小鼠学习记忆能力的影响及其机制。方法以SAMP8小鼠为研究对象,给予葡萄内酯,连续灌胃45 d。采用Morris水迷宫方法测定小鼠学习记忆能力,用比色法测定脑组织超氧化物歧化酶(SOD)和乙酰胆碱酯酶(AChE)活力、丙二醛(MDA)和谷氨酸(Glu)含量。结果葡萄内酯中、高剂量组到达平台的时间明显缩短,与模型对照组比较,差异有统计学意义(P<0.05);葡萄内酯剂量组AChE活性、MDA和Glu含量明显降低,与模型对照组比较,差异有统计学意义(P<0.05)。结论葡萄内酯可以明显改善SAMP8小鼠学习记忆能力,其作用机制可能与抑制脑组织AChE活性有关。
Objective To investigate the effect and mechanism of grape lactone on learning and memory ability of rapidly aged mice. Methods SAMP8 mice as the research object, to give the lactone, continuous gavage for 45 days. Morris water maze method was used to determine the ability of learning and memory in mice. The contents of superoxide dismutase (SOD) and acetylcholinesterase (AChE), malondialdehyde (MDA) and glutamic acid (Glu) . Results Compared with the model control group, the time of arriving at the plateau in the medium and high dose groups of lactone was significantly shortened (P <0.05), and the activity of AChE, the content of MDA and Glu in the lactone dose group were significantly lower than those in the model control group The difference between the control group, the difference was statistically significant (P <0.05). Conclusion Vitis lactone can significantly improve the learning and memory ability of SAMP8 mice, which may be related to the inhibition of AChE activity in brain tissue.