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采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组合中选取两对引物组合EcoR I ACC+Msc I CAT和EcoR IACC+Msc I CAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个,多态性位点比例达到78.5%,对35个品种的区分率达到100%。各品种(系)多态性带数存在差别,多态性带数最多为海南红蕉(56条),多态性比例最高为0.666 7,可见其杂合程度较高;多态性带最少的为大丰1-1号(33条),其多态性比例为0.392 9。各品种(系)相互之间的相似系数介于0.71~1.00,表明香牙蕉品种(系)之间遗传关系相对较近。依相似系数0.88的水平,可以将供试的35个香牙蕉品种(单株)分为6个品种群。鉴别了3个引自不同国家的品种,几内亚、苹果以及阳江矮实际上为同一个香牙蕉品种。该研究结果基于AFLP分子标记,能从分子水平上反映香牙蕉地方品种之间的亲缘关系,可以作为香牙蕉品种(系)分类的依据。
AFLP technique was used to identify and classify 35 varieties of Canna indica. AFLP analysis was performed on 35 samples of Aralia elata from two pairs of primer combinations, EcoR I ACC + Msc I CAT and EcoR IACC + Msc I CAG, from 64 pairs of primers provided by AFLP kit. A total of 107 amplification sites were obtained There were 84 polymorphic loci with 78.5% of polymorphic loci and 100% of 35 cultivars. There was a difference in the number of polymorphic bands among the cultivars (strains). The maximum number of polymorphic bands was Hainan Red Banana (56), the highest proportion of polymorphic bands was 0.666 7, indicating a higher degree of heterozygosity; the least polymorphic bands For the Dafeng 1-1 (33), the proportion of 0.392 9 polymorphism. The similarity coefficients of the varieties (lines) were between 0.71 and 1.00, indicating that the genetic relationship between varieties was relatively close. According to the similarity coefficient of 0.88, 35 varieties of canna indica (single plant) can be divided into six groups. Three varieties from different countries were identified, Guinea, Apple and Yangjiang Dwarf actually being the same fragrant banana varieties. Based on the AFLP molecular markers, the results of this study can reflect the genetic relationship between local banana cultivars at the molecular level and can be used as the basis for the classification of banana cultivars.