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目的: 探讨中国HIV/AIDS患者HIV -1Gag、Tat、Rev和Nef蛋白特异性CTL应答的特征。方法: 应用覆盖HIV -1B、C亚型Gag、Tat、Rev和Nef蛋白的 220个肽段作为抗原, 通过ELISPOT方法检测HIV/AIDS患者HIV特异性CTL应答。结果: 无论HIV- 1B亚型还是HIV- 1C亚型所构建肽库的应答强度和频率, 主要集中在Gag和Nef蛋白, Tat和Rev蛋白也有不同程度的应答。HIV- 1B、C亚型间应答比较,整体应答强度大致相同, 但免疫优势区间存在着一定的差异,B亚型Gagp24亚蛋白的 288 ~313氨基酸区应答最强, 而C亚型Gagp24亚蛋白的 155 ~181氨基酸区应答最强; 两个亚型免疫优势区应答频率最高的都是Nef蛋白 106~143氨基酸区(48. 1% )。结论: 中国人群CTL应答多集中在Gag和Nef蛋白, B、C亚型间略有差异且存在交叉识别, 这对设计针对中国人群的HIV疫苗是有重要的意义。
Objective: To investigate the characteristics of HIV-1 Gag, Tat, Rev and Nef protein-specific CTL responses in HIV / AIDS patients in China. Methods: The HIV-specific CTL responses of HIV / AIDS patients were detected by ELISPOT method using 220 peptides covering the HIV-1 B, C subtypes Gag, Tat, Rev and Nef proteins as antigens. Results: The strength and frequency of peptide libraries constructed by either HIV-1B subtypes or HIV-1C subtypes were mainly concentrated in Gag and Nef proteins. Tat and Rev proteins also responded to varying degrees. The responses of HIV-1B and C subtypes were similar, but the overall response strengths were about the same, but there were some differences in the immunodominant regions. The subtype Gagp24 of subtype B had the strongest response in the region of 288-313 amino acids, whereas the subtype Gagp24 of subtype C Of the amino acid region of the highest response to 155-181; two subtypes immunodominant region highest response frequency is Nef protein 106 to 143 amino acid region (48.1%). CONCLUSION: CTL responses in Chinese population are mostly concentrated on Gag and Nef proteins. There is a slight difference between B and C subtypes and cross-recognition, which is of great significance for designing HIV vaccines targeting at Chinese population.