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为了解全基因组水平上绿豆类甜蛋白家族基本生物学特征,通过生物信息学手段,鉴定绿豆类甜蛋白家族成员,并对基因和蛋白结构及染色体定位进行了分析,为进一步克隆绿豆类甜蛋白基因以及探讨其抗真菌活性奠定基础。结果表明:共鉴定出34个绿豆类甜蛋白家族成员,基因主要有4种结构类型,分布在除4号和9号染色体外的其余9条染色体中。该家族蛋白功能主要构成细胞结构组分和参与细胞生物学进程。系统发育分析显示,绿豆类甜蛋白家族归属10个聚类组,其中成员较多的聚类组6和聚类组7中的基因主要来自11号染色体,其中7个基因簇中的基因存在紧密连锁现象,属于旁系同源基因,可能发生了染色体内复制。密码子使用性分析显示,绿豆TLP家族基因偏好A或T作为第三位密码子,但密码子使用总体偏性不强,多数属于低表达基因,多数基因进化受碱基突变和正向选择压力的双重影响。
In order to understand the basic biological characteristics of the mung bean sweet protein family at genome-wide level, the mung bean sweet protein family members were identified by means of bioinformatics analysis. The gene and protein structure and chromosome location were analyzed. In order to further clone mung bean sweet protein Gene and explore its antifungal activity. The results showed that 34 members of mung bean sweet protein family were identified. There are four main types of genes in the protein family, which are distributed in the remaining 9 chromosomes except chromosomes 4 and 9. The family of proteins functions primarily as cellular components and participates in cell biology processes. Phylogenetic analysis showed that the mung bean sweet protein family belongs to 10 cluster groups, of which cluster 6 and cluster 7 with more members mainly come from chromosome 11, among which the genes in 7 cluster are close Chain phenomenon, belonging to paralogous genes, may have occurred in the chromosomal duplication. Codon usage analysis showed that mung bean TLP family gene preferred A or T as the third codon, but the overall bias of codon usage was not strong, most of which belonged to low expression genes. Most of the genes evolved by base mutation and positive selection pressure Double impact.