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Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) Merrill.Most effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L~(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.
Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica (Burm. Fil.) Merrill. Motive effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere- The seeds of similar morphology, was achieved through 75 mmol·L -1 calcium chloride (CaCl_2 · 2H_2O) plus 3% (w / v) Na-alginate with 93.3% conversion frequency. The earliest conversion (within 7 days of incubation ) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) ° C, (15 ± 1) ° C, and (25 ± 1) ° C] Artificial seeds at (15 ± 1) ° C executed the highest frequency of conversion (90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at (15 ± 1) ° C was recorded at 70% without further dec The present protocol could be expedient for short-term storing and swapping of T. The present protocol may be expedient for short-term storage and swapping of T. indica germplasms between national and international laboratories.