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【目的】研究不同条件下复合菌系的秸秆分解能力和酶活性,评价该复合菌系的功能稳定性及适应性,为进一步完善该复合菌系的筛选技术及其应用提供理论依据。【方法】以复合菌系GF-20为研究对象,在不同温度和pH值条件下连续继代培养,测定所得复合菌系的滤纸分解特性、玉米秸秆降解率、发酵液pH值、纤维素酶和半纤维素酶活性,以评价复合菌系的功能稳定性。【结果】在温度4~30℃、pH 6.0~9.0时,经过15代的继代培养,复合菌系GF-20在培养的108~140h可分解滤纸纤维素,玉米秸秆降解率保持在30%以上,发酵液pH值随发酵时间的延长趋近于中性,pH调节能力良好,纤维素酶活性为1.22~2.34 U/mL,半纤维素酶活性为12.82~14.23U/mL。【结论】复合菌系GF-20可在较大的温度和pH范围内保持较高的纤维素和半纤维素酶活性,能发挥秸秆降解作用,具有良好的开发潜力和应用前景。
【Objective】 The objective of this study was to study the straw decomposition and enzyme activities of composite strains under different conditions and to evaluate the functional stability and adaptability of the composite strains for providing a theoretical basis for further screening and application of the composite strains. 【Method】 The complex strain GF-20 was used as the research object, under the condition of different temperature and pH, the cells were subcultured successively to determine the characteristics of filter paper decomposition, degradation rate of corn stalks, pH value of fermentation broth, cellulase And hemicellulase activity to evaluate the functional stability of the complex strains. 【Result】 The results showed that the degradation rate of corn straw was 30% at the temperature of 4 ~ 30 ℃, pH 6.0 ~ 9.0, after 15 generations of subculture, the composite strain GF-20 could decompose filter paper cellulose in 108 ~ 140h. The pH value of fermentation broth was close to neutral with the prolongation of fermentation time, and the pH adjusting ability was good. The cellulase activity was 1.22-2.34 U / mL and the hemicellulase activity was 12.82-14.23U / mL. 【Conclusion】 The composite strain GF-20 can maintain high cellulase and hemicellulase activity in a wide range of temperature and pH, exert the effect of straw degradation, and has good development potential and application prospects.