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目的 探讨P16蛋白在肝细胞癌(HCC)中的表达及其临床意义。方法 应用免疫组织化学技术(S-P法)检测68例肝细胞癌(HCC)中P16蛋白的表达。同时进行增殖细胞核抗原(PCNA)检测,计算细胞增殖指数(proliferation index.PI)。结果 HCC中P16蛋白阳性表达率为42.7％,而癌旁肝组织及肝硬化组织P16蛋白阳性表达率分别为94.7％和90.0％。HCC P16阳性表达率明显低于癌旁组织及肝硬化组织(P<0.01)。P16蛋白低表达与HCC临床分期,组织分化程度及细胞增殖指数相关(P<0.05)。结论 P16蛋白低表达有促进HCC增殖的作用,可作为HCC恶性程度的一项指标。
Objective To investigate the expression and clinical significance of P16 protein in hepatocellular carcinoma (HCC). Methods The expression of P16 protein in 68 cases of hepatocellular carcinoma (HCC) was detected by immunohistochemical technique (S-P method). Proliferating cell nuclear antigen (PCNA) was also measured and the proliferation index (PI) was calculated. Results The positive expression rate of P16 protein in HCC was 42.7%, while the positive expression rate of P16 protein in para-carcinoma tissues and cirrhotic tissues was 94.7% and 90.0%, respectively. The positive rate of HCC P16 expression was significantly lower than that of paracancerous tissue and cirrhotic tissue (P<0.01). The low expression of P16 protein correlated with the clinical stage, histological differentiation and cell proliferation index of HCC (P<0.05). Conclusion The low expression of P16 protein can promote the proliferation of HCC, which can be used as an indicator of the malignancy of HCC.