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目的在基因转录水平了解核糖体相关基因在大鼠再生肝(RL)和肝脏肿瘤(LT)中的表达异同。方法用搜索网站资料和查阅相关论文等方法获得核糖体发生、组装和功能相关基因及它们在肝脏肿瘤中的表达变化,用大鼠基因组230 2.0芯片检测它们在大鼠再生肝中的表达情况,用统计学方法比较上述基因在再生肝和肝脏肿瘤中的表达异同。结果初步证实上述基因中75个基因与肝再生相关。其中,18个基因在再生肝和肝脏肿瘤中表达上调,6个基因在两者中表达下调,51个基因在肝脏肿瘤中未发生有意义的表达变化。结论再生肝的核糖体相关基因表达谱与肝脏肿瘤有相同之处,也有不同之处,前者的基因表达更具阶段性和复杂性。
Objective To understand the similarities and differences in the expression of ribosome related genes in rat regenerative liver (RL) and liver tumor (LT) at the gene transcription level. Methods The expression of ribosomes, assembly and function related genes and their expression in liver tumors were obtained by means of searching website materials and consulting related theses. The rat genome 230 2.0 was used to detect their expression in rat regenerative liver. Statistical comparison of these genes in regenerative liver and liver tumors in the expression of similarities and differences. The results initially confirmed that 75 of the above genes were associated with liver regeneration. Among them, 18 genes were upregulated in regenerative liver and liver tumors, 6 genes were down-regulated in both, and 51 genes had no significant changes in liver tumors. Conclusions The expression pattern of ribosome related genes in regenerative liver is similar to that of liver tumors. There are also differences between the two. The former has more stage and complex gene expression.