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目的:采用基因表达谱芯片技术筛选疏风宣肺解毒方治疗流感病毒感染小鼠相关的差异表达基因,初步探讨方药治疗流感的作用机制。方法:流感病毒肺适应株FM1滴鼻感染小鼠,建立小鼠流感病毒性肺炎模型,方药灌胃治疗4 d,提取肺组织总RNA,与小鼠全基因表达谱芯片杂交后进行检测。对筛选得到的药物治疗过程中的差异表达基因进行生物信息学分析。结果:基因芯片结果显示,与模型组相比,给药组表达量发生变化的基因中上调的有2 659条,下调的有2 216条。结论:疏风宣肺解毒方作用涉及免疫学方面的分子功能为:胞内信号级联放大效应,浆膜、免疫应答、细胞增生的调节,免疫防御及细胞程序性死亡等;主要调节的代谢途径有:丝裂原活化蛋白激酶信号通路、细胞因子与细胞因子受体的相互作用、肿瘤信号通路、toll样受体信号通路、信号转导及转录激活因子途径、自然杀伤细胞介导的细胞毒作用及趋化因子信号通路等。
OBJECTIVE: To screen the differentially expressed genes associated with influenza virus infection in mice by using Shufeng Xuanfei Jiedu Decoction gene therapy by gene expression microarray, and to explore the mechanism of action of traditional Chinese medicine in the treatment of influenza. Methods: The mouse model of influenza virus pneumonia was established by intranasal inoculation of influenza virus lung-adapted strain FM1. Four days after intragastric administration, the total RNA was extracted from the lung tissue and hybridized with mouse whole gene expression microarray. The bioinformatics analysis of differentially expressed genes in the drug treatment process was carried out. Results: The result of gene chip showed that compared with the model group, there were 2 659 up-regulated genes in gene expression group and 2 216 down-regulated genes. Conclusion: Shufeng Xuanfei Jiedu Prescription has immunological molecular functions such as amplification of intracellular signaling cascade, serosal regulation, immune response, regulation of cell proliferation, immune defense and programmed cell death Pathways include: Mitogen-activated protein kinase signaling pathway, cytokine-cytokine receptor interaction, tumor signaling pathway, toll-like receptor signaling pathway, signal transduction and transcriptional activator pathway, natural killer cell-mediated cell Toxicity and chemokine signaling pathways.