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目的探讨AITD与个体携带HLA-DQA1*0301、HLA-DQA1*0501、DQA1*0201等位基因的相关性。方法对自身免疫性甲状腺病的GD患者194例、HT患者118例和非自身免疫性甲状腺病的SG患者85例以及健康者148例,采用PCR-SBT方法扩增HLA-DQA1*0301、HLA-DQA1*0501、DQA1*0201目的基因片段,进行率的χ2检验,计算OR值,对比分析他们等位基因分布频率的差异,探讨他们之间的相关性。结果 194例GD患者和118例HT患者的HLA-DQA1*0301、HLA-DQA1*0501等位基因分布频率均显著高于对照组,OR值均>1;HLA-DQA1*0201等位基因分布频率则明显低于对照组,OR值均<1。而非自身免疫性的SG患者的HLA-DQA1*0301、HLA-DQA1*0501、DQA1*0201与对照组之间无显著性差异。结论携带HLA-DQA1*0301、HLA-DQA1*0501等位基因的个体与GD患者和HT患者的发病易感性有相关性,而携带DQA1*0201等位基因的个体则与该病保护性相关。
Objective To investigate the association between AITD and individuals carrying HLA-DQA1 * 0301, HLA-DQA1 * 0501 and DQA1 * 0201 alleles. Methods 194 patients with autoimmune thyroid disease GD, 118 patients with HT and 85 patients with non-autoimmune thyroid disease SG and 148 healthy subjects, using PCR-SBT method to expand HLA-DQA1 * 0301, HLA- DQA1 * 0501, DQA1 * 0201 gene fragments, the rate of χ2 test, calculate the OR value, comparative analysis of their allele frequency distribution differences, to explore the correlation between them. Results The frequencies of HLA-DQA1 * 0301 and HLA-DQA1 * 0501 alleles in 194 patients with GD and 118 patients with HT were significantly higher than those in the control group (OR> 1). The frequencies of HLA-DQA1 * 0201 alleles Was significantly lower than the control group, OR values were <1. There was no significant difference between HLA-DQA1 * 0301, HLA-DQA1 * 0501, DQA1 * 0201 and the control group in patients with autoimmune SG. Conclusion Individuals carrying alleles of HLA-DQA1 * 0301 and HLA-DQA1 * 0501 are associated with the susceptibility to developing disease in GD patients and HT patients, while individuals carrying DQA1 * 0201 allele are protectively associated with the disease.