作者用免疫组织化学分析几个角质化(KC)肿瘤和炎性皮肤疾病中bcl-2蛋白表达,研究良性和恶性皮肤肿瘤发展中bcl-2的作用。 方法:用抗-bcl-2单克隆抗体的免疫染色检查72例冰冻组织标本,取自炎性KC增殖患者(慢性皮肤病[CD])和普通银屑病[PV],皮脂溢角质化病[SK]和原发性KC肿瘤病(急性角质化病[AK]和Bowen’s病[BD]),基底细胞癌(BCC)和扁平细胞癌(SCC),2株SCC细胞系,以及20例正常皮肤细胞。 结果:正常的KC、CD、PR组织和SK组织呈现 The authors used immunohistochemistry to analyze bcl-2 protein expression in several keratinized (KC) tumors and inflammatory skin diseases to study the role of bcl-2 in the development of benign and malignant skin tumors. METHODS: Seventy-two frozen tissue specimens were examined by immunostaining with anti-bcl-2 monoclonal antibodies and were obtained from patients with inflammatory KC proliferation (chronic dermatosis [CD]) and ordinary psoriasis [PV], seborrheic keratosis [SK] and primary KC oncology (acute keratinization [AK] and Bowen’s disease [BD]), basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), two SCC cell lines, and 20 normal Skin cells. Results: Normal KC, CD, PR and SK tissues were present