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目的:研究新疆多伞阿魏提取物对胃癌细胞株SGC-7901增殖抑制作用及作用机制。方法:采用二苯基四氮唑溴盐比色法(MTT法)检测新疆多伞阿魏不同提取物对SGC-7901细胞增殖的抑制作用,筛选出抑制作用最强的提取物W3。光镜下观察W3不同浓度对SGC-7901细胞形态的影响,TUNLE凋亡染色实验检测W3诱导SGC-7901细胞凋亡作用,流式细胞仪进一步检测其对细胞凋亡率的影响。结果:多伞阿魏提取物W3能显著抑制SGC-7901细胞增殖,同时具有诱导细胞凋亡作用,且呈剂量依赖关系。结论:多伞阿魏提取物W3抑制胃癌细胞增殖作用可能与诱导细胞凋亡有关。
OBJECTIVE: To study the inhibitory effect and the mechanism of U. polyphemus extract on the proliferation of gastric cancer cell line SGC-7901. Methods: The inhibitory effect of different extracts of Radix Ulexisensis on the proliferation of SGC-7901 cells was detected by diphenyl tetrazolium bromide colorimetry (MTT method), and the strongest extract W3 was screened out. The effect of different concentrations of W3 on the morphology of SGC-7901 cells was observed under light microscope. The apoptosis of SGC-7901 cells was detected by TUNLE staining, and the effect of W3 on cell apoptosis was detected by flow cytometry. Results: W3C can inhibit the proliferation of SGC-7901 cells and induce apoptosis in a dose-dependent manner. CONCLUSION: W3 can inhibit the proliferation of gastric cancer cells through inducing cell apoptosis.