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目的研究Mn Cl2对生精细胞天冬氨酸特异性的半胱氨酸蛋白酶-3(caspase-3)和增殖细胞核抗原(PCNA)表达的影响,探讨Mn诱发大鼠生精细胞caspase-3和PCNA表达的机制及Zn的拮抗作用。方法雄性SD大鼠80只随机分为空白对照组,Zn Cl2对照组,15和30 mg/kg Mn Cl2组;15和30 mg/kg Mn Cl2+Zn Cl2组。Mn Cl2组染Mn 4和6周,Zn Cl2对照组和Mn+Zn组始终或染Mn 4周后给予100 mg Zn2+/kg饲料2周。采用免疫组织化学法检测睾丸caspase-3和PCNA表达。结果各染Mn组、Mn+Zn组及染Mn剂量相同的6周组,染Mn时间相同的30 mg/kg Mn Cl2组caspase-3阳性细胞率均显著升高,Mn+Zn组显著降低。染Mn 4周PCNA阳性细胞率显著降低,染Mn 6周组和Mn+Zn组由于残存细胞数量减少反而升高。结论染Mn15 mg/kg 4周可诱发大鼠生精细胞caspase-3表达且抑制PCNA表达,且随染Mn时间和剂量的增加而增加,两者分别存在一定时间-效应和剂量-效应关系;摄入含Zn100 mg/kg的食物可拮抗Mn诱发生精细胞caspase-3表达。
Objective To investigate the effects of Mn Cl2 on the expression of caspase-3 and PCNA in aspartate-derived spermatogenic cells, and to explore the effects of MnCl on the expression of caspase-3 Mechanism of PCNA Expression and Zn Antagonism. Methods Eighty male SD rats were randomly divided into blank control group, Zn Cl2 control group, 15 and 30 mg / kg MnCl2 group, 15 and 30 mg / kg Mn Cl2 + Zn Cl2 group. MnCl 4 and 6 weeks were stained with Mn Cl 2, and Zn Cl 2 control and Mn + Zn were given with 100 mg Zn 2 + / kg diet for 4 weeks or 4 weeks after Mn administration for 2 weeks. Immunohistochemistry was used to detect the expression of caspase-3 and PCNA in testis. Results The caspase-3 positive rate in Mn group, Mn + Zn group and Mn group with the same dose of Mn at 30 mg / kg Mn Cl2 for the same Mn time were significantly increased, and Mn + Zn group was significantly decreased. The rate of PCNA-positive cells was significantly decreased after 4 weeks of Mn exposure, while the levels of PC 6 and Mn + Zn in Mn-Zn group increased with the decrease of the number of remaining cells. Conclusion Mn15 mg / kg for 4 weeks induces the expression of caspase-3 and inhibits the expression of PCNA in rat spermatogenic cells, and increases with the time and dosage of Mn. There is a time-effect and dose-effect relationship between the two. Dietary Zn up to 100 mg / kg could antagonize Mn-induced caspase-3 expression in spermatogenic cells.