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目的探讨ATP结合盒(ABC)转运体ABCG2,ABCB5在皮肤黑素瘤中的共表达及意义。方法采用免疫组化单/双重染色法检测30例皮肤黑素瘤、30例皮内痣及5例A375细胞移植瘤中ABCG2,AB-CB5的表达与共表达。结果①ABCG2在黑素瘤、皮内痣中阳性率分别为63.33%和0,两者相比差异有统计学意义(P<0.05),阳性细胞率平均值分别为(25.50±9.47)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(27.00±8.04)%;②ABCB5在黑素瘤、皮内痣中阳性率分别为73.33%和0,两者相比差异有统计学意义(P<0.05),阳性细胞率平均值分别为(28.75±2.22)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(30.50±4.66)%;③ABCG2和ABCB5在黑素瘤、皮内痣中共表达阳性率分别为60.00%和0,两者相比差异有统计学意义(P<0.05),共表达阳性细胞率平均值分别为(23.00±2.94)%和0,前者明显高于后者,在A375细胞移植瘤中阳性率和阳性细胞率平均值分别为100.00%和(24.50±4.04)%。结论皮肤黑素瘤与A375细胞移植瘤中存在ABCG2,ABCB5的阳性表达和共表达,提示皮肤黑素瘤的多药耐药可能与ABCG2和ABCB5有关。
Objective To investigate the expression and significance of ATP-binding cassette ABCG2 and ABCB5 in cutaneous melanoma. Methods The expressions of ABCG2 and AB-CB5 in 30 melanoma, 30 intradermal nevus and 5 A375 cell xenografts were detected by immunohistochemical single / double staining. Results ① The positive rates of ABCG2 in melanoma and neoplasm were 63.33% and 0 respectively, the difference was statistically significant (P <0.05), the positive rate of positive cells were (25.50 ± 9.47)% and , The former was significantly higher than the latter in the A375 cell xenografts in the positive rate and the average rate of positive cells were 100.00% and (27.00 ± 8.04)%; ②ABCB5 in melanoma, intradermal nevus positive rates were 73.33% (P <0.05). The positive rate of positive cells were (28.75 ± 2.22)% and 0 respectively, the former was significantly higher than the latter The positive rates of ABCG2 and ABCB5 in melanoma and neoplasm were 60.00% and 0%, respectively (P <0.05). The positive rates of positive cells were 100.00% and (30.50 ± 4.66)%, respectively <0.05). The positive rates of co-expressing positive cells were (23.00 ± 2.94)% and 0, respectively. The former was significantly higher than the latter. The positive rates of positive cells and positive cells in A375 cells were 100.00% and 24.50 ± 4.04)%. Conclusion The positive expression and co-expression of ABCG2 and ABCB5 exist in skin melanoma and A375 cell xenografts, suggesting that multidrug resistance of cutaneous melanoma may be related to ABCG2 and ABCB5.