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本研究采用膜片钳全细胞记录技术记录低渗诱发的人低分化鼻咽癌细胞(CNE-2Z)钾电流,用图像分析技术测量细胞容积,探讨不同类型K+通道在调节性容积回缩(regulatory volume decrease,RVD)中的作用。结果显示,细胞外灌流160mOsm/L低渗液诱发CNE-2Z细胞产生容积激活性钾电流和RVD,中等电导钙依赖性钾通道阻断剂clotrimazole(100μmol/L)几乎完全抑制低渗诱发的钾电流和RVD。去除细胞外液的Ca2+后,低渗刺激不能诱发钾电流,RVD显著减弱。电压依赖性钾通道阻断剂4-aminopyridine(4-AP,5mmol/L)部分抑制低渗诱发的钾电流。上述结果提示,钙依赖性钾通道是低分化鼻咽癌细胞容积激活性钾通道的主要类型;钙依赖性钾通道在鼻咽癌细胞RVD中发挥了关键性作用,而电压依赖性钾通道在其中起部分作用。
In this study, patch-clamp whole-cell recording technique was used to record the potassium currents in human hypotonic nasopharyngeal carcinoma cells (CNE-2Z) induced by hypotonicity. The cell volume was measured by image analysis technique and the effects of different types of K + regulatory volume decrease, RVD). The results showed that volume-activated K + current and RVD were induced in CNE-2Z cells by extracellular perfusion of 160mOsm / L hypothages, while clotrimazole (100μmol / L), a medium-conductance calcium-dependent potassium channel blocker, Current and RVD. Removal of extracellular Ca2 +, hypotonic stimulation can not induce potassium current, RVD was significantly weakened. The voltage-dependent potassium channel blocker 4-aminopyridine (4-AP, 5 mmol / L) partially suppresses hypotonic-induced potassium currents. These results suggest that calcium-dependent potassium channel is the main type of low-differentiated NPC cell volume-activated potassium channels; calcium-dependent potassium channels play a key role in the RVD of nasopharyngeal carcinoma cells, and voltage-dependent potassium channels in Which play a part.