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把响叶杨（Ｐｏｐｕｌｕｓａｄｅｎｏｐｏｄａ）优树叶片接种在ＷＰＭ＋６－ＢＡ０．５～１ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ培养基上培养，在叶表面产生大量不定芽。将带２个腋芽的嫩茎切段接种在１／２ＭＳ＋６－ＢＡ０．２～０．５ｍｇ／Ｌ＋ＮＡＡ０．０１ｍｇ／Ｌ的培养基上培养，在腑芽抽长的同时，从基部和抽长的部位分化出许多不定芽。将这两类不定芽转移到附加ＮＡＡ０．０１ｍｇ／Ｌ和１．５％蔗糖的１／２ＭＳ培养基上培养可形成生根小植株。生根小植株经炼苗后移栽到盛混合基质（１／３火土＋１／３田园土＋１／３腐熟厩肥）的营养钵中，成活率达８０％以上。组培苗定植到田间后生长比稼接苗快。
The leaves of Populus adenopoda were inoculated on the medium of WPM + 6-BA0.5 ~ 1mg / L + NAA1mg / L to produce a large number of adventitious buds on the leaf surface. The shoots with two axillary buds were inoculated on medium of 1 / 2MS + 6-BA0.2 ~ 0.5mg / L + NAA0.01mg / L and cultured. Differentiation of many adventitious buds. These two types of adventitious buds were transferred to 1/2 MS medium supplemented with NAA 0.01 mg / L and 1.5% sucrose to form rooted plantlets. The rooted plantlets were transplanted into a nutrient bowl of Sheng mixed base (1/3 fire earth +1/3 pasture soil + 1/3 decomposed manure fertilizer) after the seedling was harvested, and the survival rate was over 80%. Tissue culture seedlings planted in the field after growing faster than Jia then Miao.