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目的探讨细胞周期蛋白Cyclin Y在直肠癌组织中的异常表达及其临床意义。方法收集2014年7月至2015年6月72例行直肠癌根治术的患者组织标本,采用免疫组织化学法检测Cyclin Y的表达情况,30例距肿瘤5 cm以上的直肠黏膜组织作为正常直肠黏膜作实验对照。统计方法采用SPSS 20.0统计软件处理,Cyclin Y蛋白表达率及其与各病理因素的关系采用卡方检验、Kruskal-Wallis检验等方法分析,以P<0.05为差异具有统计学意义。结果 Cyclin Y蛋白在直肠癌细胞中的表达定位以细胞膜和胞质为主。Cyclin Y在直肠正常黏膜中表达阳性率为43.3%(13/30),在直肠癌组织中表达阳性率为80.5%(58/72),各组之间相比差异有统计学意义(P<0.05)。Cyclin Y蛋白在直肠癌中的表达水平与患者的性别、年龄、家族史、肿瘤大小及浸润深度无关(P>0.05),而与淋巴结转移、直肠癌病理分期有显著相关性(P<0.05)。结论 Cyclin Y在直肠癌中存在过表达现象,可能在直肠癌发生发展过程中起重要的调节作用。
Objective To investigate the abnormal expression of cyclin Y in rectal cancer and its clinical significance. Methods Tissue specimens from 72 patients with rectal cancer undergoing radical resection from July 2014 to June 2015 were collected. The expression of Cyclin Y was detected by immunohistochemical method. Thirty patients with rectal mucosa more than 5 cm away from the tumor were selected as normal rectal mucosa For experimental control. Statistical methods SPSS20.0 statistical software processing, Cyclin Y protein expression and its relationship with the various pathological factors using chi-square test, Kruskal-Wallis test and other methods to P <0.05 was considered statistically significant. Results The expression of Cyclin Y protein in rectal cancer cells was mainly located in the cell membrane and cytoplasm. The positive rate of Cyclin Y was 43.3% (13/30) in rectal mucosa and 80.5% (58/72) in rectal cancer tissues, the difference was statistically significant (P < 0.05). The expression level of Cyclin Y protein in rectal cancer was not correlated with the gender, age, family history, tumor size and depth of invasion (P> 0.05), but significantly correlated with lymph node metastasis and pathological stage of rectal cancer (P <0.05) . Conclusion Cyclin Y is overexpressed in rectal cancer and may play an important regulatory role in the development of rectal cancer.