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实验以玉米田黑土为研究对象,主要研究土壤氮素转化功能菌及其活性在不同施入量农肥和化肥处理下的变化。结果表明:农肥高量处理使土壤氨化细菌和硝化细菌数量升高,分别是对照的2.22倍和4.09倍,而化肥高量处理使反硝化细菌数量明显增加,是对照的8.49倍;农肥处理对土壤微生物量碳、微生物量氮、土壤脲酶和土壤过氧化氢酶有促进作用,其活性明显高于对照。相关分析表明,土壤微生物量碳与土壤脲酶,土壤微生物量氮与过氧化氢酶和土壤氨化细菌之间均存在着极显著的正相关关系,而土壤微生物量碳与土壤氨化细菌之间存在着极显著的负相关关系。
In the experiment, the black soil in corn field was taken as the research object, and the changes of functional nitrogen-transmutating bacteria and their activities under the different manure and fertilizer treatments were studied. The results showed that high amount of fertilizer increased soil ammonifier and nitrobacteria, which were 2.22 times and 4.09 times that of the control respectively. However, high amount of chemical fertilizer increased the number of denitrifying bacteria significantly, which was 8.49 times that of the control. Soil microbial biomass carbon, microbial biomass nitrogen, soil urease and soil catalase had a promoting effect, the activity was significantly higher than the control. Correlation analysis showed that there was a significant positive correlation between soil microbial biomass C and soil urease, soil microbial biomass nitrogen and catalase and soil ammonification bacteria, but there was a significant positive correlation between soil microbial biomass carbon and soil ammonification bacteria There is a very significant negative correlation.