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目的:优选分离冬凌草多糖的方法,探究不同分离方法对冬凌草多糖分离效果的影响。方法:分别以DEAE-Sepharose Fast Flow(Cl-型)弱极性阴离子交换层析法、乙醇分级醇析法对冬凌草粗多糖进行分离纯化,以不同分离组分中多糖含量及其免疫活性比较优选冬凌草粗多糖的分离工艺。结果:DEAE-Sepharose Fast Flow(Cl-型)弱极性阴离子交换层析法分离得到5个组分,通过乙醇分级醇沉初步得到4个冬凌草多糖组分,前者分离产品纯度较高,而后者产品在体外小鼠脾淋巴T细胞的增殖实验中表现出较强生物活性。结论:为进一步研究冬凌草多糖的生物活性,可选用乙醇分级醇沉法对冬凌草多糖进行分离纯化。
Objective: To optimize the separation of Rubescens polysaccharides, and to explore the different isolation methods on Rubescens polysaccharide separation effect. Methods: The crude polysaccharides of Rubia cordifolia were isolated and purified by DEAE-Sepharose Fast Flow (Cl-type) weakly polar anion exchange chromatography and ethanol fractionation. The polysaccharide contents and immunological activities The more preferred Rubescens crude polysaccharide separation process. Results: Five components were isolated by anion exchange chromatography with weak polarities, DEAE-Sepharose Fast Flow (Cl-type). Four fractions of Rubescens indica polysaccharides were obtained by ethanol fractionation. The latter product showed strong biological activity in the proliferation experiments of mouse splenic lymphocyte T cells in vitro. Conclusion: To further study the bioactivity of Rubescens polysaccharides, ethanol grade alcohol precipitation method can be used to isolate and purify Rubescens polysaccharides.