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[目的]探讨痛泻要方对醋酸-电刺激大鼠实验性肠易激综合征(IBS)的作用及其机制。[方法]给予大鼠慢性反复直结肠灌注醋酸造成炎症刺激,炎症恢复后予小量电刺激诱发高敏感性的肠道发生应激反应,模拟人类IBS,观察痛泻要方对刺激期间大鼠排便量、粪便含水量、血浆P物质(SP)和血管活性肠肽(VIP)水平等各项指标的影响。[结果]模型组与正常组比较,大鼠排便量、粪便含水量增加(P<0.01),血浆SP和VIP减少(P<0.05);痛泻要方高剂量组与模型组比较,大鼠的排便量、粪便含水量减少(P<0.05),血浆SP和VIP增加(P<0.05)。[结论]醋酸-电刺激大鼠模型可有效模拟人类IBS,痛泻要方能够改善该模型的排便加速、粪便含水量增加、血浆SP和VIP水平紊乱等症状。
[Objective] To explore the effect and mechanism of Tongxie Yaofang on acetic acid-electrical stimulation of experimental irritable bowel syndrome (IBS) in rats. [Methods] Rats were given acetic acid to induce inflammatory stimuli in chronic rectal colon repeatedly. After recovery of inflammation, a small amount of electrical stimulation was used to induce high-response stress response in the intestine to simulate human IBS. Observing the effect of Tongxie Yaofang on rats during the stimulation period. Defecation volume, fecal moisture content, plasma substance P (SP) and vasoactive intestinal peptide (VIP) levels and other indicators. [Results] Compared with the normal group, the amount of defecation and fecal water in the model group increased (P<0.01), and the SP and VIP decreased in the model group (P<0.05); the high-dose group of Tongxiyokefang group compared with the model group. Defecation volume and fecal water content decreased (P<0.05), plasma SP and VIP increased (P<0.05). [Conclusion] Acetic acid-electrically stimulated rat model can effectively imitate human IBS, and Tongxie Yaofang can improve the symptoms of defecation acceleration, increase of fecal water content, disorder of plasma SP and VIP levels and other symptoms.