论文部分内容阅读
目的探讨生长抑素受体(SSTR)显像对乳腺癌的诊断价值。方法 15例乳腺癌患者术前进行99mTc-奥曲肽SSTR显像,勾画感兴趣区,计算瘤体与对侧正常组织的放射性比值(T/N), 术后采用逆转录-聚合酶链反应检测肿瘤组织SSTR1-SSTR5 mRNA的表达,SP免疫组化法比较该 15例乳腺癌和18例乳腺良性病变组织SSTR2和SSTR5的表达水平。结果①15例乳腺癌患者 99mTc-奥曲肽SSTR显像均为阳性,T/N为1．873±0．341;②15例乳腺癌组织的SSTR1、SSTR2、 SSTR3、SSTR4、SSTR5mRNA表达阳性率分别为100％(15/15)、93．30％(14/15)、100％(15/15)、 46．70％(7/15)、86．70％(13/15),以甘油醛-3-磷酸脱氢酶(GAPDH)基因为内参基因计算的mRNA 表达量,从高到低分别为SSTR3、SSTR1、SSTR2、SSTR5、SSTR4,SSTR2 mRNA表达水平与乳腺癌显像T/N呈正相关,有显著的统计学意义(r=0．728,p<0．01);③SP免疫组化结果显示,乳腺癌组织的SSTR2和SSTR5的表达明显高于乳腺良性病变组织。结论乳腺癌组织有丰富的SSTR 表达,明显高于乳腺良性病变组织,99mTc-奥曲肽SSTR显像对乳腺癌有较好的靶向诊断价值。
Objective To investigate the diagnostic value of somatostatin receptor (SSTR) imaging in breast cancer. Methods Totally 99mTc-octreotide SSTR imaging was performed in 15 breast cancer patients. The region of interest was plotted to calculate the ratio of tumor to normal tissue (T / N). Tumors were detected by reverse transcription-polymerase chain reaction The expression of SSTR1-SSTR5 mRNA was detected by immunohistochemistry and the expression of SSTR2 and SSTR5 in the 15 cases of breast cancer and 18 cases of benign breast lesions. Results ① The 99mTc-octreotide SSTR imaging was positive in all 15 breast cancer patients with a T / N ratio of 1.873 ± 0.341. The positive rates of SSTR1, SSTR2, SSTR3, SSTR4 and SSTR5 mRNA in 15 breast cancer tissues were 100% (15/15), 93.30% (14/15), 100% (15/15), 46.70% (7/15), 86.70% (13/15) The expression of SSTR3, SSTR1, SSTR2, SSTR5, SSTR4 and SSTR2 mRNAs from high to low were positively correlated with the imaging T / N of breast cancer (R = 0.728, p <0.01). ③ SP immunohistochemical results showed that the expression of SSTR2 and SSTR5 in breast cancer tissues was significantly higher than that in benign breast lesions. Conclusion The expression of SSTR in breast cancer tissues is significantly higher than that in benign breast lesions. The 99mTc-octreotide SSTR imaging has a good target value in the diagnosis of breast cancer.