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采用传统的饱和酚提取法和上海生物工程公司的UN IQ-10柱式基因组DNA抽提试剂盒法对大弹涂鱼的总DNA进行了提取。琼脂糖凝胶电泳结果表明,得到的DNA片段分子量在21 kb以上;通过PCR扩增实验,证明了用两种方法提取的DNA均可直接用于随机扩增的DNA多态性(RAPD)遗传标记;试剂盒法具有简单、快速、高效的优点,是一种值得推荐的DNA提取方法。
Total saturated DNA was extracted by traditional saturated phenol extraction method and Shanghai Bioengineering Company’s UN IQ-10 column genomic DNA extraction kit method. The results of agarose gel electrophoresis showed that the molecular weight of the obtained DNA fragment was over 21 kb. The PCR amplification experiments showed that the DNA extracted by both methods can be directly used for random amplified polymorphic DNA (RAPD) Labeling; kit method has the advantages of simple, fast and efficient, is a recommended method of DNA extraction.