论文部分内容阅读
利用野生广东石斛种子,经无菌播种在MS+香蕉70 g/L+AC 3 g/L+蔗糖30 g/L,琼脂7.0 g/L,pH 5.4,种子萌发后形成大量PLBS需要110 d左右。这些PLBS在培养基MS+BA 0.1 mg/L+NAA 0.2 mg/L上繁殖系数为6.0以上。而PLBS在培养基1/4MS+香蕉70 g/L+AC 1 g/L分化成细小植株后,再经1/2MS+香蕉70 g/L+NAA 0.5 mg/L+AC 0.3 g/L培养后形成完整植株。播种苗在温室中用水草种植其成活率100%。
Seeds of wild Dendrobium candidum in Guangdong were sown aseptically for about 110 days after MS / banana 70 g / L + AC 3 g / L + sucrose 30 g / L, agar 7.0 g / L, pH 5.4. These PLBSs had a reproductive coefficient of 6.0 or higher in medium MS + BA 0.1 mg / L + NAA 0.2 mg / L. However, PLBS was cultured in 1 / 4MS + banana with 70 g / L and 1 g / L of banana after being differentiated into small plants and then cultured with 1 / 2MS + banana 70 g / L + NAA 0.5 mg / L + AC 0.3 g / Complete plant. Sowing seedlings planted in the greenhouse with aquatic plants its survival rate of 100%.