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本研究鉴定水稻基因组中类甜蛋白(TLP)家族基因,并对部分基因的组织表达特异性进行研究,为开展水稻类甜蛋白基因功能及分子进化机制研究提供基础。基于水稻基因组数据库,通过生物信息学方法对候选的47个水稻类甜蛋白基因家族成员进行鉴定、聚类及结构功能分析,通过q RT-PCR技术,检测14个Os TLP基因在LTH及其近等系水稻幼苗的叶片、根、叶鞘和花穗中的时空表达水平。基因和蛋白结构分析表明,Os TLP基因有5种结构类型,蛋白保守氨基酸基序高度一致。聚类分析显示,水稻类甜蛋白基因归属10个进化组,分布于12条染色体上,其中成员最多的进化组Ⅴ中的基因主要来自3号和12号染色体。q RT-PCR结果显示,14个Os TLP在6个品种水稻根、叶鞘、叶片和花穗中相对表达水平不同,多个基因在根中有较高的基础表达。本研究为进一步研究TLP家族在水稻生长发育和对抗胁迫中的作用提供了理论基础。
In this study, we identified TLR family genes in rice genome and studied the tissue expression specificity of some genes in order to provide a basis for studying the function of TLR genes and molecular evolution mechanism in rice. Based on the rice genomic database, 47 candidate members of the rice thaumatin gene family were identified, clustered and analyzed by bioinformatics methods. The qRT-PCR technique was used to detect 14 Os TLP genes in LTH and its vicinity Isochronous rice seedling leaves, roots, leaf sheaths and flower spike in the spatial and temporal expression levels. Structural analysis of genes and proteins showed that there are five structural types of Os TLP gene, and the protein conserved amino acid motifs are highly consistent. Cluster analysis showed that the sweet-thaw genes in rice belonged to 10 evolutionary groups distributed on 12 chromosomes. The most abundant members of evolutionary group Ⅴ mainly came from chromosomes 3 and 12. q RT-PCR results showed that the relative expression levels of 14 Os TLPs in 6 varieties of rice roots, leaf sheaths, leaves and flower spikes were different, and multiple genes were highly expressed in roots. This study provides a theoretical basis for further study on the role of TLP family in rice growth and development and the fight against stress.