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目的:观察濒危药用植物川东獐牙菜主要提取物芒果苷与苦苷协同对HepG2人肝癌细胞的作用。方法:在体外培养的人肝癌细胞中依次加入50μg/ml川东獐牙菜水提物和乙醇不同萃取部位,在镜下观察HepG2人肝癌细胞生存状态。以芒果苷和苦苷标准品为对照,用MTT法研究正丁醇部位提取物的OD值。结果:发现正丁醇部位提取的芒果苷和苦苷的含量最高,正丁醇部位提取物作用HepG2人肝癌细胞24小时后,胞质出现空泡,细胞漂浮死亡。MTT检测其OD值呈线性递增关系,即芒果苷与苦苷混合物对肝癌细胞的杀灭作用最明显,并呈浓度依赖性。结论:濒危药用植物川东獐牙菜正丁醇部位提取物芒果苷与苦苷对体外人肝癌细胞有明显抑制和杀灭作用,其协同作用优于芒果苷或苦苷分别作用。
Objective: To observe the effect of the synergistic effect of mangiferin and matrine on the HepG2 human hepatoma cells. Methods: HepG2 human hepatocarcinoma cells were cultured in vitro with 50μg / ml water extract of Swertia cordata and ethanol extract. The survival of HepG2 human hepatocellular carcinoma cells was observed under microscope. To manganoside and the standard product of matrine for the control, MTT method for the study of n-butanol extract of the OD values. Results: The content of mangiferin and bitter glycoside extracted from the n-butanol fraction was found to be the highest. 24 hours after the extraction of n-butanol fraction from HepG2 human hepatocellular carcinoma cells, the cytoplasm vacuolized and the cells died. MTT test showed a linear increasing relationship between the OD value, that is, the mixture of mangiferin and bitterness had the most obvious killing effect on hepatocarcinoma cells in a concentration-dependent manner. CONCLUSION: Mangiferin and bitter glycosides, which are extracts from n-butanol of endangered medicinal plant Swertia brasiliensis, can inhibit and kill human hepatocellular carcinoma cells in vitro. The synergistic effect is better than that of mangiferin or bitterness.