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目的:观察桑枝对小鼠脑缺血再灌注损伤的保护作用。方法:将小鼠分为4组,假手术组;模型组(分离两侧颈总动脉及迷走神经,用微型动脉夹同时夹闭双侧颈总动脉,制作小鼠脑缺血再灌注模型);阳性药组给以尼莫地平24mg/kg,受试药组给以桑枝水提醇沉液14g生药/kg,均灌胃给药7天,24h后取材,匀浆,测脑组织Evan’s蓝含量及抗氧化酶SOD活性与MDA水平的变化。结果:缺血再灌注后,脑组织Evan’s蓝含量升高,给药后降低。脑缺血再灌注组SOD活性降低,MDA含量增高,而治疗组SOD活性增高,MDA水平降低。结论:桑枝能增加脑缺血再灌注小鼠SOD的活性,改善脑血流,减轻血脑屏障的损伤,对脑缺血再灌注损伤具保护作用。
Objective: To observe the protective effect of mulberry branches on cerebral ischemia-reperfusion injury in mice. Methods: The mice were divided into 4 groups: sham operation group, model group (carotid artery and vagus nerve on both sides were separated, bilateral common carotid arteries were occluded with micro-artery clip, and the model of cerebral ischemia / reperfusion was made). Positive drug group to nimodipine 24mg / kg, the experimental group to give mulberry water extract alcohol sediment 14g crude drug / kg, were gavage for 7 days, 24h after drawing, homogenate, measuring brain tissue Evan’s blue Content and activities of antioxidant enzymes SOD and MDA levels. Results: After ischemia-reperfusion, the content of Evan’s blue in brain increased and decreased after administration. Cerebral ischemia-reperfusion group, SOD activity decreased, MDA content increased, while the treatment group increased SOD activity, MDA decreased. Conclusion: Sangzhi can increase the activity of SOD in cerebral ischemia-reperfusion mice, improve cerebral blood flow, alleviate the damage of the blood-brain barrier and protect the cerebral ischemia-reperfusion injury.