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以铁皮石斛种荚为外植体,研究了不同消毒方式、培养基配方对于外植体生长的影响,探索最适于原球茎分化诱导、壮苗生长和生根的培养基配方。结果表明:有利于原球茎分化诱导的培养基配方是1/2 MS+2.0mg/L BA+0.2mg/L NAA;适合壮苗生长的培养基配方是MS+3.0mg/L BA+1.0mg/L NAA+1.0mg/L KT+3%香蕉;适合幼苗生根的培养基配方是1/2MS+0.5mg/L NAA+3%香蕉+0.5%活性碳。
Taking Dendrobium officinale as explants, the effects of different disinfection methods and medium formulas on the growth of explants were studied, and the best medium for protocorm differentiation induction, seedling growth and rooting was explored. The results showed that medium supplemented with protocorm-like medium was 1/2 MS + 2.0 mg / L BA + 0.2 mg / L NAA, and medium supplemented with MS + 3.0 mg / L BA + 1.0 mg / L NAA + 1.0mg / L KT + 3% banana; medium suitable for seedling rooting is 1 / 2MS + 0.5mg / L NAA + 3% banana + 0.5% activated carbon.