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随着转基因棉花种植面积的日益增加,棉铃虫Helicoverpa armigera(Hbner)对Bt的抗性已经成为一个不容忽视的问题。发展转多价基因作物是当前缓解害虫对Bt抗性的最有效措施。本研究以经室内多年筛选的、抗性倍数达2000多倍的Bt杀虫剂(含多种蛋白)抗性品系为材料,通过生物测定和不同的杂交试验,测定棉铃虫对Bt杀虫剂的抗性遗传方式,以期为Bt生物农药的抗性治理提供一定的依据,同时为制定棉铃虫对转多基因作物的抗性治理策略提供一定的参考。对敏感亲本和抗性亲本杂交产生的F1代的研究结果表明,杂交品系的抗性倍数分别为22.2倍和24.6倍;抗性显性度D值均小于0,分别为-0.20和-0.17,抗性为常染色体不完全隐性遗传。对4种回交后代和2种自交后代F2的研究结果表明,实际死亡率与期望死亡率差异较大,说明抗性是由单基因多个位点或多基因控制。
With the growing area of transgenic cotton, the resistance of Helicoverpa armigera (Hbner) to Bt has become a problem that can not be ignored. The development of multi-gene transgenic crops is the most effective measure to alleviate the resistance of pests to Bt. In this study, Bt insecticides (including a variety of protein) resistant strains screened for many years in indoor and multiples of resistance multiples of 2000 were used as biomaterials and different hybridization assays to determine the effects of Bt insecticides In order to provide a basis for the resistance management of Bt biological pesticides and to provide some reference for the development of resistance management strategies for transgenic cotton bollworm. The results of F1 generation from the crosses between susceptible and resistant parents showed that the resistance multiples of hybrid lines were 22.2 and 24.6 fold, respectively; the resistance dominance values were all less than 0 (-0.20 and -0.17, respectively) Resistance is an autosomal recessive inheritance. The results of F2 progenies and 4 backcross progenies showed that there was a large difference between actual mortality and expected mortality, indicating that resistance is controlled by multiple loci or polygenes.