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目的：建立一种直接分离测定体液中庆大霉素、丁胺卡那霉素、妥布霉素、西梭霉素和乙基西梭霉素等５种氨基苷类抗生素的高效液相色谱－间接光度检测（ＨＰＬＣ－ＩＰＤ）法。方法：在流动相中加入具有紫外检测响应的检测剂烟酰胺，用紫外检测器直接测定紫外吸收很差的上述５种药物。Ｃ１８固定相，流动相为含烟酰胺０５ｍｍｏｌ·Ｌ－１、庚烷磺酸钠５ｍｍｏｌ·Ｌ－１和磷酸００５ｍｏｌ·Ｌ－１的甲醇－乙腈－水（２７∶１８∶５５）混合溶液。结果：血清和尿样平均回收率均大于９６％，日内和日间ＲＳＤ均小于６％。并测定了肌注此类药物病人的血清和尿样品。结论：该法适于体液中氨基苷类药物检测。
OBJECTIVE: To establish a HPLC method for the direct separation and determination of 5 aminoglycoside antibiotics, such as gentamycin, amikacin, tobramycin, sisamycin and ethylthromycin in body fluids Indirect Photometric Detection (HPLC-IPD). Methods: Nicotinamide, a detector with UV detection response, was added to the mobile phase. The UV absorbers were used to directly determine the above five drugs with poor UV absorption. C18 stationary phase and the mobile phase consisted of methanol-acetonitrile-water (27:18:55) containing 0.5 mmol·L-1 nicotinamide, 5 mmol·L-1 heptane sodium sulfonate and 005 mol·L-1 phosphate. mixture. Results: The average recoveries of serum and urine samples were both higher than 96%. The intra-day and inter-day RSDs were less than 6%. Serum and urine samples from patients with intramuscular injections of these drugs were also measured. Conclusion: This method is suitable for the detection of aminoglycosides in bodily fluids.