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在昆明市文心兰栽培基地发现了具有病毒病症状的感病文心兰和大花惠兰植株。感病文心兰和大花惠兰样品在电镜下可观察到长约460-500 nm的线状病毒粒体和长约300 nm的杆状病毒粒体,它们分别与已报道的建兰花叶病毒和齿兰环斑病毒粒体大小一致。利用ELISA技术在感病文心兰和大花惠兰样品可以检测到建兰花叶病毒和齿兰环斑病毒。利用建兰花叶病毒和齿兰环斑病毒的特异性引物,运用RT-PCR方法可以扩增到与引物设计预期大小一致的核酸条带。由此证明,云南文心兰和大花惠兰病毒病由建兰花叶病毒和齿兰环斑病毒中的一种或两种侵染引起。
In the Wen Xinlan cultivation base in Kunming found a virus disease symptoms of Sickleinensis and Da Hua Hui Lan plants. Sensein and Cymbidium grandiflora samples were observed under electron microscopy linear virus mitochondria about 460-500 nm in length and about 300 nm in length of the baculovirus virus, respectively, they have been reported to build Orchid mosaic The size of the virus and toxin ring spot virus are the same. Construction of Orchid Mosaic Virus and Tequila Spot Virus could be detected by ELISA in susceptible and turbinate samples. By using the specific primers of Glandula mosaic virus and Toothpider annulada virus, a nucleic acid band which is consistent with the expected size of the primer can be amplified by RT-PCR. This proves that Yunnan Oncidium and Grandiflora virus disease caused by one or both of the Cymbidium mosaic virus and the Tequila annua virus.