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目的:观察芪术颗粒在肝纤维化形成过程中对p38 MAPK信号传导通路的影响。方法:Waster大鼠随机分为正常组、模型组、实验对照组和芪术颗粒组,用四氯化碳复制肝纤维化模型,造模同日即给予相应治疗,分别于造模后的第1、2、4周处理大鼠,取肝组织Western blot免疫印迹法检测总p38 MAPK、p-p38 MAPK、总ATF-2及p-ATF-2蛋白表达。结果:随着肝纤维化的进展,总p38 MAPK、p-p38 MAPK、总ATF-2及p-ATF-2蛋白表达均有上调,而芪术颗粒对其上调均有一定的抑制作用,与模型组比较差异显著(P<0.05)。结论:芪术颗粒对p38 MAPK信号传导通路的激活有调控作用,从而抑制肝纤维化的发生和发展。
Objective: To observe the effect of Qizhu granule on the p38 MAPK signal transduction pathway in the process of hepatic fibrosis. Methods: Wistar rats were randomly divided into normal group, model group, experimental control group and Qizhu granule group. Carbon tetrachloride was used to replicate hepatic fibrosis model. The model rats were given the same treatment on the same day. The rats were sacrificed at 2 and 4 weeks. The expression of total p38 MAPK, total ATF-2 and p-ATF-2 protein in liver tissue was detected by Western blot. Results: With the progress of hepatic fibrosis, the expressions of total p38 MAPK, p-p38 MAPK, total ATF-2 and p-ATF-2 protein were all up-regulated, The model group had significant difference (P <0.05). Conclusion: Qizhu granule can regulate the activation of p38 MAPK signal pathway and thus inhibit the occurrence and development of hepatic fibrosis.