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已有研究表明,吗啡依赖大鼠脊髓能够提高培养背根节细胞的存活率。为了从形态学证实吗啡依赖大鼠脊髓对在体背根节细胞的影响,取10只大鼠分为吗啡备用根组和备用根对照组。术前给予实验动物递增性注射吗啡,使其成瘾,术后继续给予维持量吗啡。切除两组动物一侧L_1～L_3和L_5～L_6背根和背根节,保留L_4背根(备用根)。术后34天灌注固定动物,取L_4脊髓后角,制作包括一侧完整Ⅱ板层的超薄切片,在电镜(×7000)下抽样摄片,观察计数照片范围(107μm~2)内来自背根的复合终末(CT)和非背根来源的简单终末(ST)数,并测量CT的面积。结果显示:1.两组动物手术侧和非手术侧Ⅱ板层横断面积及其神经毡面积分数没有差别,这为手术侧和非手术侧Ⅱ板层相同单
Studies have shown that morphine-dependent rat spinal cord can increase the survival rate of cultured dorsal root ganglion cells. In order to confirm morphine-dependent morphine-dependent rat spinal cord on the dorsal root ganglion cells, 10 rats were divided into morphine standby root group and spare root control group. Morphine was given to experimental animals incrementally before surgery to addiction, and morphine was continuously administered after the operation. L_1 ~ L_3 and L_5 ~ L_6 dorsal root and dorsal root ganglion were excised from the two groups of animals, while L_4 dorsal root (reserve root) was preserved. The fixed animals were perfused 34 days after operation, and the posterior horn of L4 spinal cord was taken out to make an ultrathin section including the complete lamina of one side. Samples were taken under electron microscope (× 7000) and observed in the range of 107μm ~ 2 Root complex terminal (CT) and non-dorsal root derived simple terminal (ST) number, and measure the area of CT. The results showed that: 1. There was no difference between the two groups in the cross-sectional area of the operative and non-surgical side Ⅱ laminae and their neuroflagellate fraction, which were the same as the non-surgical lamina Ⅱ