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介绍一种用原位杂交组织化学与免疫组织化学结合法在同一切片中显示生长抑素mRNA和相对应的生长抑素.同时还比较了六种不同固定液对原位杂交组织化学和原位杂交组织化学与免疫组织化学结合法的影响.结果表明Zamboni氏固定液用于原位杂交组织化学与免疫组织化学结合法最佳,而4%多聚甲醛磷酸缓冲液用单纯原位杂交最佳.原位杂交组织化学与免疫组织化学结合法显示的下丘脑室周核生长抑素免疫反应阳性神经元均含有相应生长抑素mRNA,而且多数神经元生长抑素基因的转录量与生长抑素的翻译量成正比.
In situ hybridization histochemistry and immunohistochemistry were used to show the somatostatin mRNA and its corresponding somatostatin in the same section, and the effect of six different fixatives on in situ hybridization histochemistry and in situ Hybrid tissue chemistry and immunohistochemistry.The results showed that Zamboni’s fixative was the best for in situ hybridization and immunohistochemistry, and 4% paraformaldehyde phosphate buffer was best by simple in situ hybridization The in situ hybridization histochemistry and immunohistochemical staining showed that somatostatin immunoreactive neurons in periventricular nucleus of hypothalamus all contained the corresponding somatostatin mRNA, and most of the neuronal somatostatin gene transcription and somatostatin The amount of translation is proportional to.