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目的:探讨川芎-天麻配伍对血瘀型偏头痛的治疗作用及不同配比的疗效差异性。方法:SD雄性大鼠42只,随机分为正常组(Z),模型组(M),假手术组(J),川芎-天麻(4∶1)组(A),川芎-天麻(1∶1)组(B),川芎-天麻(1∶4)组(C),舒马曲坦组(阳性组,Y)。A,B,C 3组大鼠,将川芎-天麻浓缩液按剂量20 m L·kg~(-1)·d~(-1),连续灌胃8 d,Z,J组将舒马曲坦水溶液按20 m L·kg~(-1)·d~(-1),连续灌胃8 d。大鼠冰水浴后皮下注射盐酸肾上腺素(0.8 mg·kg~(-1))和硝酸甘油(10 mg·kg~(-1))复制血瘀证偏头痛模型,采用酶联免疫吸附(ELISA)检测各组大鼠血清中降钙素基因相关肽(CGRP),血栓烷素B2(TXB_2),6-酮-前列腺素F1α(6-Keto-PGF1α)和脑组织一氧化氮(NO),一氧化氮合酶(NOS),降钙素基因相关肽(CGRP),内皮素(ET)水平。结果:与正常组比较,模型组血清中NO,NOS,CGRP,6-Keto-PGF1α含量,NO/ET明显升高(P<0.05);脑组织中NO,NOS,ET,CGRP,TXB_2,6-Keto-PGF1α含量明显升高(P<0.05);各比例组比较,川芎-天麻(4∶1)组能显著降低模型组血清中CGRP,6-Keto-PGF1α含量,NO/ET(P<0.05),提高TXB_2含量,TXB_2/6-Keto-PGF1α(P<0.05);(4∶1)组显著降低模型动物脑组织中NO,ET,TXB_2,6-Keto-PGF1α含量与NOS活性,提高TXB_2/6-Keto-PGF1α(P<0.05)。结论:川芎-天麻配伍对治疗血瘀型偏头痛具有疗效,不同配比组疗效有差异,综合多项指标考虑,川芎-天麻(4∶1)组疗效最好。
Objective: To investigate the therapeutic effect of rhizoma chuanxiong - gastrodia tuber mix on migraine with blood stasis and the curative effect difference of different proportions. METHODS: Forty-two SD male rats were randomly divided into normal group (Z), model group (M), sham operation group (J), Chuanxiong-Tianma (4:1) group 1) group (B), Chuanxiong - Gastrodia (1: 4) group (C), sumatriptan group (positive group, Y). Rats in group A, B and C were treated with 20 m L · kg -1 d -1 of Chuanxiong-Tianma Concentrate for 8 days, The solution of tamsulosin was infused at 20 m L · kg -1 (-1) d -1 for 8 days. The rat model of migraine induced by blood stasis was subcutaneously injected with epinephrine hydrochloride (0.8 mg · kg -1) and nitroglycerin (10 mg · kg -1) after ice-water bath. ELISA and enzyme-linked immunosorbent assay (CGRP), TXB_2, 6-Keto-PGF1α and brain tissue nitric oxide (NO) were determined in each group. NOS, CGRP and ET. Results: Compared with normal group, NO, NOS, CGRP, 6-Keto-PGF1α and NO / ET in model group were significantly increased (P <0.05); NO, NOS, ET, CGRP, TXB_2, The content of Keto-PGF1αin the model group was significantly lower than that in the control group (P <0.05), and the content of CGRP and 6-Keto-PGF1α in the model group was significantly lower than that of the Chuanxiong-Tianma (4:1) TXB2, 6-Keto-PGF1α (P <0.05); (4:1) significantly decreased the levels of NO, ET, TXB_2, 6-Keto-PGF1α and the activity of NOS in model animals TXB_2 / 6-Keto-PGF1α (P <0.05). Conclusion: Rhizoma Chuanxiong - Gastrodia elata rhizome compatibility has a curative effect on the treatment of migraine with blood stasis syndrome, and there are differences in the curative effects between different prescription groups. Considering a number of indexes, Chuanxiong - Tianma (4:1) group has the best curative effect.