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采用沙土水溶液的培养方法,以3个主栽大豆品种合丰50、合丰55和绥农28为试验材料,分别在种子发芽期(VE期)、第二片三出复叶长出期(V2期)进行不同浓度的NaCl处理,测定种子发芽率、死亡叶面积率、相对株高盐害率等表型指标,检测丙二醛含量、相对电导率等耐盐性生理指标。数据分析表明,该方法能区分不同品种、不同处理浓度的盐害程度,反映出相同品种、不同处理浓度间的差异,证明该方法能够用于大豆耐盐性鉴定。通过该鉴定体系测定3个大豆品种的耐盐性,最终选出在VE期抑制50%种子发芽NaCl浓度、V2期死亡叶面积率和对照之间存在显著差异的最小NaCl筛选浓度,并把V2期的死亡叶面积率作为衡量耐盐性的主要表型指标,丙二醛含量作为主要生理指标。研究结果可用于大豆耐盐性的鉴定、转基因耐盐大豆的鉴定等领域,具有重要应用价值。
The three main cultivated soybean varieties Hefeng 50, Hefeng 55 and Suinong 28 were used as experimental materials in the process of seed germination (VE) V2) were treated with different concentrations of NaCl. The seed germination rate, the area of dead leaves and the salt damage rate of relative plants were measured. The physiological indexes of salt tolerance such as MDA content and relative conductivity were detected. The data analysis shows that the method can distinguish the salt damage degree of different varieties and different treatment concentrations, and reflect the difference among the same varieties and different treatment concentrations, which proves that the method can be used to identify the salt tolerance of soybean. The salt tolerance of three soybean cultivars was determined by the identification system. Finally, the minimum NaCl concentration that could inhibit the seed germination of NaCl at 50%, the leaf area at the stage of V2 death and the control at the VE stage was selected and the concentration of V2 As the main phenotypic index of salt tolerance, the MDA content of dead leaves was the main physiological index. The research results can be used to identify the salt tolerance of soybean and the identification of transgenic salt-tolerant soybean, which has important application value.