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目的探讨siRNA沉默Msi1基因表达对人胃癌SGC-7901细胞增殖、凋亡、侵袭与转移的影响。方法针对Msi1 mRNA序列设计合成siRNA,转染SGC-7901细胞。RT-PCR法检测Msi1基因表达;Westernblot检测survivin、caspase3及MMP-9表达;MTT法检测SGC-7901细胞增殖;流式细胞术检测细胞凋亡情况。结果Msi1 siRNA能有效抑制胃癌SGC-7901细胞中Msi1的表达,与空白对照组比较,Msi1 siRNA组SGC-7901细胞生长、增殖速度减缓(P<0.05);survivin、MMP9蛋白表达水平显著下调(P<0.05),而caspase3蛋白表达水平上调(P<0.05);Msi1 siRNA组凋亡率增高。结论沉默Msi1对人胃癌SGC-7901细胞增殖、凋亡、侵袭与转移有负性调节作用。
Objective To investigate the effects of siRNA silencing Msi1 gene on the proliferation, apoptosis, invasion and metastasis of human gastric cancer cell line SGC-7901. Methods siRNA targeting siRNA Msi1 was transfected into SGC-7901 cells. The expression of Msi1 gene was detected by RT-PCR, the expression of survivin, caspase3 and MMP-9 were detected by Western blot, the proliferation of SGC-7901 cells was detected by MTT assay, and the apoptosis was detected by flow cytometry. Results Msi1 siRNA could effectively inhibit the expression of Msi1 in gastric cancer SGC-7901 cells. Compared with the blank control group, the growth and proliferation rate of SGC-7901 cells in Msi1 siRNA group were significantly decreased (P <0.05), while the expressions of survivin and MMP9 were significantly decreased <0.05), while the expression of caspase3 protein was up-regulated (P <0.05). The apoptosis rate of Msi1 siRNA group was increased. Conclusion Silencing Msi1 can negatively regulate the proliferation, apoptosis, invasion and metastasis of human gastric cancer cell line SGC-7901.