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从化学、仿生物、细胞三个层次,水溶、脂溶两种体系研究了脂溶性茶多酚(LTP)的抗氧化特性,并与维生素E(VE ),绿茶多酚(GTP)等进行了比较⒚结果表明:对水溶性体系中的·OH,巨噬细胞呼吸爆发产生的氧自由基的清除效果为GTP> LTP> VE⒚对大分子稳定的二苯基苦基肼基(DPPH)自由基,LTP,GTP效果接近且均显著优于VE⒚在膜体系中,GTP对Fe2+ 诱导的膜脂质过氧化的抑制作用优于LTP,VE⒚而VE 对2,2'偶氮2,4二甲基戊腈(AMVN)诱导的膜脂质过氧化的抑制作用显著优于LTP, GTP,当浓度大于50 μg/m L时,LTP的抑制作用保持在25% 左右并显著优于GTP⒚LTP基本保留了原有茶多酚优良的抗氧化特性,在脂溶性体系中效果较好⒚同时给出了抗氧化剂在各体系中 50%抑制率或清除率时的浓度,即IC50 或SC50⒚
The antioxidative properties of liposoluble tea polyphenols (LTP) were studied from the chemical, biological and cellular levels, water-soluble and fat-soluble systems. The antioxidative properties of LTP were also studied with vitamin E (VE), green tea polyphenols The results showed that the scavenging effect on oxygen free radicals produced by respiratory burst of · OH and macrophages in water-soluble system was GTP> LTP> VE⒚, which could stabilize macromolecules of diphenyl picrylhydrazyl (DPPH) free radical , The effects of LTP and GTP are both close to that of VE. In the membrane system, GTP has better inhibitory effect on Fe2 + -induced membrane lipid peroxidation than LTP, VE and VE on 2,2’-azo-2,4 The inhibition of membrane lipid peroxidation induced by dimethylvaleronitrile (AMVN) was significantly better than that of LTP and GTP. When the concentration was higher than 50 μg / mL, the inhibitory effect of LTP remained at about 25% and was significantly better than that of GTP⒚LTP Retain the original antioxidant properties of tea polyphenols in the fat-soluble system better ⒚ at the same time The concentration of antioxidant at 50% inhibition or clearance in each system, ie IC50 or SC50⒚