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[背景]虽然来自一般人群中男性的数据有限,但持久性环境化学物和精液质量之间关系的研究仍在不断发展之中。[目的]研究全氟化学物(PFCs)和501对计划怀孕的夫妇中男性伴侣的精液质量之间的关系。[方法]采用基于人群的抽样策略,从2005—2009年在两个美国地理区域内招募了501对停止避孕措施的夫妇。首先进行基线采访和人体测量学评估,随后采集血液样本,用串联质谱法定量7种血清全氟化学物(全氟磺酸、全氟羧酸和全氟磺酰胺)的含量。在基线期和约1个月后收集男性的精液样本。精液样本用冰袋贮存并运送,并在收集后的第二天进行分析。用线性回归估计自然对数转换的PFC浓度每增加一个单位各精液参数的差异,分析中校正了混杂因素,并用重复测量的精液样本建模。敏感性分析包括采用最优的Box-Cox转换精液质量终点指标。[结果]6种PFCs[2-(N-甲基全氟辛烷亚磺酰氨基)乙酸乙酯(Me-PFOSA-Ac OH)、全氟癸酸甲酯(PFDe A)、全氟壬酸甲酯(PFNA)、全氟辛烷磺酰胺(PFOSA)、全氟辛烷磺酸(PFOS)和全氟辛酸(PFOA)]均与Box-Cox变换前17种精液质量终点指标有关联。PFOSA与精子头部面积和周长较小、DNA染色比例较低以及双头和未成熟精子的比例更高有关联。PFDe A、PFNA、PFOA以及PFOS均与精子尾部卷曲比例较低有关联。[结论]部分全氟化物与一些精液质量终点指标有关联,PFOSA最为显著,但观察到的结果有不同。
[Background] Although data from men in the general population are limited, the relationship between persistent environmental chemicals and semen quality is still evolving. [Objective] To investigate the relationship between perfluorinated chemicals (PFCs) and 501 semen quality of male partners in couples planning pregnancy. [Method] Using a population-based sampling strategy, 501 pairs of couples who stopped contraception were recruited from 2005-2009 in two US geographical regions. Baseline interviews and anthropometric assessments were performed first, then blood samples were collected and the contents of seven serum perfluorinated chemicals (perfluorosulfonic acid, perfluorocarboxylic acid and perfluorosulfonamide) were quantified by tandem mass spectrometry. Male semen samples were collected at baseline and about 1 month later. Semen samples were stored and shipped in ice packs and analyzed on the second day after collection. Linear regression was used to estimate the difference in sperm parameters for each unit of increase in PFC concentration from natural logarithmic conversions, with confounding factors corrected for in the analysis and modeled with replicate semen samples. Sensitivity analysis includes using the optimal Box-Cox to translate seminal quality endpoint. [Results] Six kinds of PFCs [Me-PFOSA-AcOH, PFDe A, perfluorononanoic acid (PFNA), PFOSA, PFOS and PFOA were correlated with the 17 semen quality indicators before Box-Cox transformation. PFOSA is associated with smaller sperm head area and circumference, lower DNA staining, and a higher proportion of double-headed and immature sperm. PFDe A, PFNA, PFOA, and PFOS were associated with lower percentages of sperm tail curl. [Conclusion] Some perfluorinated compounds are related to some end-point indicators of semen quality. PFOSA is the most significant, but the observed results are different.