论文部分内容阅读
目的建立LC-MS/MS法同时测定大鼠血浆中芍药苷和芍药内酯苷的含量,并研究灌胃给药四物汤后芍药苷和芍药内酯苷在大鼠体内的药代动力学性质。方法大鼠血浆样品用乙腈沉淀蛋白;以甲醇和0.1%甲酸-水溶液为流动相梯度洗脱,用色谱柱Thermo Syncronis C18(2.1 mm×100 mm,5μm)进行分离;定量分析采用API 4000质谱仪的多反应监测扫描方式、负离子模式检测。将建立的方法应用于大鼠口服四物汤后芍药苷和芍药内酯苷的药代动力学研究。药代动力学参数由WinNonlin 5.2统计软件进行计算。结果芍药苷和芍药内酯苷分别在2~1000μg/L和1~1000μg/L范围内线性关系良好,批内和批间精密度和准确度结果显示,批内、批间变异均小于15%,相对误差分别为-1.10%~2.74%和-4.02%~4.00%,方法学考察均符合要求。结论所建方法专属性强,灵敏度高,可用于芍药苷和芍药内酯苷的药动学研究。
OBJECTIVE To establish a simultaneous LC-MS / MS method for the determination of paeoniflorin and paeoniflorin in rat plasma and to study the pharmacokinetics of paeoniflorin and paeoniflorin in rats after oral administration of Siwutang nature. Methods The rat plasma samples were precipitated with acetonitrile and eluted with a mobile phase of methanol and 0.1% formic acid-water. The chromatographic separation was performed on a Thermo Syncronis C18 column (2.1 mm × 100 mm, 5 μm) Multi-reaction monitoring scanning mode, negative ion mode detection. The established method was applied to the pharmacokinetics of paeoniflorin and paeoniflorin after oral administration of Siwu Decoction in rats. Pharmacokinetic parameters were calculated by WinNonlin 5.2 statistical software. Results Paeoniflorin and paeoniflorin had a good linearity in the range of 2 ~ 1000μg / L and 1 ~ 1000μg / L, respectively. The intra-and inter-batch precision and accuracy showed that the intra- and inter-batch variations were all less than 15% , The relative errors were -1.10% ~ 2.74% and -4.02% ~ 4.00%, respectively. The methodological investigation met the requirements. Conclusion The proposed method is specific and sensitive and can be used for pharmacokinetic studies of paeoniflorin and paeoniflorin.