本研究构建了水稻Na+转运蛋白SKC1基因植物表达载体pTF-SKC1,标记基因为Bar基因。以子叶节为外植体,利用农杆菌介导法将SKC1导入大豆中。经过除草剂抗性筛选后获得的再生植株经PCR方法鉴定,转基因植株阳性率为50%,初步证明SKC1基因已整合到大豆基因组中。耐盐性试验结果表明,转基因植株的耐盐性高于对照。 In this study, a plant expression vector pTF-SKC1 for rice Na + transporter SKC1 gene was constructed and its marker gene was Bar gene. Using cotyledonary node as explants, SKC1 was introduced into soybean by Agrobacterium-mediated method. The regenerated plants obtained after herbicide resistance screening were identified by PCR method. The positive rate of transgenic plants was 50%. It was initially proved that SKC1 gene was integrated into soybean genome. Salt tolerance test results show that the salt tolerance of transgenic plants is higher than the control.