采用RAPD技术 ,以 4个小麦株高基因近等基因系 (分别含有rht、Rht1、Rht2、Rht3)为材料 ,对 2 96个单一随机引物 (10个核苷酸 )进行了筛选。发现 2 5个引物的扩增产物在近等基因系间表现出特异性。在 6次重复试验中 ,有 18个引物的特异扩增片段不能重复 ,6个引物可以重复 2～ 3次 ,唯有OPAM 0 1在全部试验中均能稳定重复 ,其特异扩增片段OPAM 0 1186 0 可以作为 rht基因的RAPD标记 A total of 2 96 single random primers (10 nucleotides) were screened by RAPD technique in four wheat near-isogenic lines (rht, Rht1, Rht2 and Rht3). The amplification products of 25 primers were found to be specific among nearly isogenic lines. In 6 replicates, 18 primers could not repeat the specific amplified fragment, 6 primers could be repeated 2 or 3 times, and only OPAM 0 1 was stable and repeatable in all experiments. The specific amplified fragment OPAM 0 1186 0 can be used as RAPD marker for rht gene