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DNA提取是分子生物学研究的基础步骤,而叶样研磨是提取步骤中最为费时费力的环节。该研究探索出一种快速、批量粉碎玉米叶片以提取DNA的方法,并以SSR分子标记试验验证此方法在分子标记辅助育种工作的应用的可行性,为大容量样品分子标记试验提供一种快速、批量、成本低、无毒安全的粉碎叶样的方法,提高试验效率。
DNA extraction is the basic step in molecular biology research, and leaf-like grinding is the most time-consuming and laborious step in the extraction step. This study explored a rapid and batch method of smashing maize leaves for DNA extraction and validated the feasibility of this method for molecular marker-assisted breeding using SSR molecular markers to provide a rapid , Batch, low cost, non-toxic and safe smash leaf-like method to improve the test efficiency.