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目的:探讨细胞培养基中不同血清比例对人胃癌细胞SGC-7901生长状态的影响,为减少培养该细胞时血清的用量。方法:用分别含有3%、6%和9%小牛血清的1640培养液分别培养SGC-7901细胞12h、24h和48h,以无血清培养基作为空白对照;倒置显微镜观察细胞的生长状况,通过基于酶标仪的MTT方法检测细胞在不同比例血清中的生长与增殖的差异。结果:SGC-7901细胞在含3%和6%小牛血清的细胞培养基中生长状态与常用的含9%小牛血清的培养基中的生长情况相比,未见显著性的差异(P>0.05);也不呈时间依赖性,但与空白血清对照组相比差异有统计学意义(P<0.05)。结论:在体外培养RPMI-1640细胞时,可将培养基中小牛血清的比例降至3%～6%,从而降低在培养该细胞过程中的的实验成本,减少不必要的血清浪费。
OBJECTIVE: To investigate the effect of different serogroups on the growth of human gastric cancer cell SGC-7901 in order to reduce the amount of serum in culture. Methods: SGC-7901 cells were cultured in 1640 medium containing 3%, 6% and 9% fetal bovine serum respectively for 12h, 24h and 48h and serum-free medium was used as blank control. The growth of SGC-7901 cells was observed by inverted microscope The MTT assay based on microplate reader was used to detect the difference of cell growth and proliferation in different proportion of serum. RESULTS: There was no significant difference in the growth status of SGC-7901 cells in 3% and 6% fetal bovine serum medium compared to the usual medium containing 9% fetal bovine serum (P > 0.05), but also time-dependent, but there was significant difference compared with blank serum control group (P <0.05). CONCLUSION: In vitro culture of RPMI-1640 cells reduces the proportion of bovine serum in the medium to 3% to 6%, thereby reducing the experimental cost of culturing the cells and reducing unnecessary serum wastes.