咖啡属植物含有相当数量的咖啡因,它对人体有刺激作用。许多与咖啡因有关的健康问题,仍在谨慎研究中。鉴于咖啡因危害健康和精神治疗作用的争论,故开展了咖啡因的生物合成和新陈代谢变化、运输和积累、遗传控制、染色体断裂和精神治疗作用等许多方面的研究。据报道,咖啡因含氮26.38％。在小粒神咖啡和中粒种咖啡叶片中和生殖器官中的咖啡因与氮含量呈正相关。据报道,咖啡因的合成随施氮量的增加而增加。本试验的目的在于探讨叶片咖啡因和含氮量的相关性,并研究在收获季节叶片的咖啡因、氮和干物质含量的变化。为了弄清生殖发育对叶片的干物质积累、咖啡因合成和氮含量的影响,须按花芽萌发、花芽发育、开花、浆果的不同发育期和收获等阶段,分期采集叶片样品。表1列述了各发育阶段和采样的详情。 Caffeine contains a considerable amount of caffeine, which has a stimulating effect on the human body. Many caffeine-related health problems are still under study. In view of the controversy over the health and psychotherapeutic effects of caffeine, many aspects of caffeine biosynthesis and metabolism, transport and accumulation, genetic control, chromosomal rupture and psychotropic effects have been studied. It is reported that caffeine nitrogen content of 26.38%. There was a positive correlation between caffeine and nitrogen content in small and medium-sized coffee leaves and in reproductive organs. It has been reported that the synthesis of caffeine increases with the amount of nitrogen applied. The purpose of this experiment was to explore the correlation between leaf caffeine and nitrogen content and to study changes in caffeine, nitrogen and dry matter content of the leaves during the harvest season. In order to understand the effect of reproductive development on leaf dry matter accumulation, caffeine synthesis and nitrogen content, leaf samples should be collected in stages according to flower germination, flower bud development, flowering, different stages of development and harvest of berry. Table 1 lists the details of each stage of development and sampling.