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以春化后但尚未抽薹的洋葱品系‘1007’的叶片为试材,采用RT-PCR结合RACE的方法克隆得到了洋葱成花素基因的cDNA全长序列,将其命名为AcFT(GenBank登录号为KF913629)。该基因cDNA全长791 bp,开放读码框为534 bp,推测其编码的蛋白由177个氨基酸残基组成,等电点为7.89,分子量为19.8 kD。将AcFT与大葱、鸢尾等植物的成花素氨基酸序列进行同源性比对,结果显示洋葱与其他植物的氨基酸序列同源性均在65%以上,其中洋葱与大葱的成花素氨基酸的同源性高达98.31%。实时荧光定量RT-PCR分析结果表明,洋葱AcFT基因在抽薹前后的根、叶鞘、叶片、假茎和花序等不同部位均有表达,尤以春化后抽薹前的叶片中表达量最高。
The full-length cDNA sequence of the oncidin gene was cloned by RT-PCR and RACE using the leaves of vernal ’1007’which had been vernalized but not yet bolted, and named it AcFT (GenBank Accession No. For KF913629). The full length cDNA of this gene is 791 bp in length and has an open reading frame of 534 bp. It is deduced that the encoded protein consists of 177 amino acid residues with an isoelectric point of 7.89 and a molecular weight of 19.8 kD. The results showed that the amino acid sequence homology between AcFT and other plants was more than 65%, and the amino acid sequence of AcFT was the same as that of scallion Source up to 98.31%. The results of real-time quantitative RT-PCR analysis showed that AcFT gene was expressed in different parts of roots, leaf sheaths, leaves, pseudostems and inflorescences before and after bolting, especially in leaves before bolting.