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目的观察淫羊藿甙对体外培养大鼠成骨细胞功能及对转录因子核心结合因子a1(Cbfa1)mRNA表达的影响,探讨淫羊藿甙抗骨质疏松的作用机制。方法用酶消化法获得新生(<24 h)SD大鼠颅盖骨成骨细胞,进行体外原代培养。将不同浓度的淫羊藿甙(1,10,100 ng/ml)分别加入培养液中,观察成骨细胞的形态、增殖和分化功能。用RT-PCR法测定成骨细胞Cbfa1、碱性磷酸酶(ALP)和Ⅰ型胶原(ColⅠ)mRNA表达水平的变化。结果 1、10和100 ng/ml淫羊藿甙均可促进Cbfa1、ALP和ColⅠmRNA表达(P<0.05),且以10 ng/ml组作用最显著。结论淫羊藿甙能促进体外培养成骨细胞增殖与分化,通过提高成骨细胞转录因子Cbfa1的基因表达水平诱导骨形成,是其抗骨质疏松作用机制之一。
Objective To observe the effect of icariine on the function of osteoblasts cultured in vitro and the expression of Cbfa1 mRNA in rats and to explore the mechanism of action of icariin on osteoporosis. Methods Newborn (<24 h) SD rat calvarial osteoblasts were obtained by enzymatic digestion and primary cultured in vitro. Different concentrations of icariin (1,10,100 ng / ml) were added to the culture medium to observe the morphology, proliferation and differentiation of osteoblasts. The changes of mRNA expression of Cbfa1, ALP and ColⅠ in osteoblasts were detected by RT-PCR. Results 1, 10 and 100 ng / ml icariin can promote Cbfa1, ALP and Col Ⅰ mRNA expression (P <0.05), and 10 ng / ml group the most significant effect. Conclusion Icariin can promote the proliferation and differentiation of cultured osteoblasts in vitro, and induce the formation of osteoblasts by increasing the gene expression of osteoblast transcription factor Cbfa1, which is one of the mechanisms of anti-osteoporosis.