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目的:对心气虚证动物模型进行研究。方法:本实验采用40只NIH小白鼠,随机分为健康对照组和心气虚模型组,用连续控食(每日50g/公斤)达到饥则损气,强迫负重游泳(10分钟/日)模拟惊劳病因,以及大剂量连续4天灌服心得安溶液0.5ml(含心得安1mg/ml)致心气耗损等综合方法造模。结果:24天后发现造模小鼠出现精神不振、活动减少、鼠尾淡白、缩肩拱背、行动迟缓、体重减轻、耐力下降,并出现气促表现,尤以强迫活动后为甚。心电图检测心率减慢,心功能检测PEP/LVET增大,EF、CO、SV、LJ值减小,心肌匀浆检测SOD活性降低,与对照组比较,差别有显著性(P<0.05)或高度显著性(P<0.01)。结论:模型基本符合心气虚证的临床表现,对心气虚证本质的探讨和寻找心气虚证的有效治疗方法具有一定的研究价值。
Objective: To study the animal model of heart qi deficiency syndrome. Methods: Forty NIH mice were randomly divided into two groups: healthy control group and model group with qi deficiency and deficiency of qi and blood, and forced weight-bearing swimming (10 minutes / day) simulation with continuous control feeding (50g / kg daily) Asthma etiology, as well as high-dose 4 days continuous infusion of propranolol solution 0.5ml (including propranolol 1mg / ml) caused by a combination of heart gas consumption and other methods. Results: After 24 days, the mice were found to be mentally retarded, decreased in activity, pale in tail, contracted shoulders, slowed in movement, decreased in weight, decreased in stamina and showed shortness of breath, especially after forced activity. The heart rate was decreased, the PEP / LVET increased, the values of EF, CO, SV and LJ decreased and the activity of SOD decreased in the myocardial homogenate. Compared with the control group, the difference was significant (P <0.05) Significance (P <0.01). Conclusion: The model is basically in line with the clinical manifestations of qi deficiency syndrome, the essence of qi deficiency syndrome and seek effective treatment of heart qi deficiency syndrome has some research value.